A composite double-/single-stranded RNA-binding region in protein Prp3 supports tri-snRNP stability and splicing

نویسندگان

  • Sunbin Liu
  • Sina Mozaffari-Jovin
  • Jan Wollenhaupt
  • Karine F Santos
  • Matthias Theuser
  • Stanislaw Dunin-Horkawicz
  • Patrizia Fabrizio
  • Janusz M Bujnicki
  • Reinhard Lührmann
  • Markus C Wahl
  • Timothy W Nilsen
چکیده

Prp3 is an essential U4/U6 di-snRNP-associated protein whose functions and molecular mechanisms in pre-mRNA splicing are presently poorly understood. We show by structural and biochemical analyses that Prp3 contains a bipartite U4/U6 di-snRNA-binding region comprising an expanded ferredoxin-like fold, which recognizes a 3'-overhang of U6 snRNA, and a preceding peptide, which binds U4/U6 stem II. Phylogenetic analyses revealed that the single-stranded RNA-binding domain is exclusively found in Prp3 orthologs, thus qualifying as a spliceosome-specific RNA interaction module. The composite double-stranded/single-stranded RNA-binding region assembles cooperatively with Snu13 and Prp31 on U4/U6 di-snRNAs and inhibits Brr2-mediated U4/U6 di-snRNA unwinding in vitro. RNP-disrupting mutations in Prp3 lead to U4/U6•U5 tri-snRNP assembly and splicing defects in vivo. Our results reveal how Prp3 acts as an important bridge between U4/U6 and U5 in the tri-snRNP and comparison with a Prp24-U6 snRNA recycling complex suggests how Prp3 may be involved in U4/U6 reassembly after splicing.

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عنوان ژورنال:

دوره 4  شماره 

صفحات  -

تاریخ انتشار 2015